Stillman Laboratory

Scope and Contents From the Sub-Series: Laboratory materials from BW Stillman and others working in his laboratory. The sub-series consists of experiments, protocols, methods, technician notes, negatives and photographic prints. Binders from BW Stillman: labelled experiments 1976-1989, Archaeal DNA Replication, Chromatin notes, Yeast, SV40, Topo S, 293 S100, RFC Sequencing. Others: Fred Bunz, Lynn Borzillo, Katie Cronin, Andrei Chabes, Gerhard Cullman, Nancy Du, Elena Ejkova, Jessica Mitchell, Patrick Finnegan, WJ Heiger,...

Scope and Contents From the Sub-Series: Laboratory materials from BW Stillman and others working in his laboratory. The sub-series consists of experiments, protocols, methods, technician notes, negatives and photographic prints. Binders from BW Stillman: labelled experiments 1976-1989, Archaeal DNA Replication, Chromatin notes, Yeast, SV40, Topo S, 293 S100, RFC Sequencing. Others: Fred Bunz, Lynn Borzillo, Katie Cronin, Andrei Chabes, Gerhard Cullman, Nancy Du, Elena Ejkova, Jessica Mitchell, Patrick Finnegan, WJ Heiger,...

Scope and Contents

A - Antigen conjugation to sepharose;

B - Prep of buffers, Blot Procedure - Formadide;

C - Calcium Procedures, Transferring DNA-CHU, Use of Bacterial Alkaline Phosphotase, Use of linkers for end structure mod., Simplified Isolation of plasmid DNA for use as restriction enzyme substrate.

Scope and Contents

D - DNA Cellulose, Purification of DNA by centrifugation (Jack Barr) 1980

E - A method for elution of polypeptides from dried polyacrylamide gels, Binding and recovery of DNA and RNA using S+S NA-45 Horizontal electrophoresis apparatus, Electron microscopy of DNA

F - Focus Mapping RNAs by digestion, 1981 A film for rapid registration of tritium

G - Gel electrophoresis, 1975

H - Restriction enzyme prep

Scope and Contents

I - Immuno ppt2 with protein A sepharose infecting fluid, In situe hybridization, Bolton Hunter reagent - New Englen Nuclear, Radioiodination od proteins in single polacrylamide gel, prep and assessment of radioactive tracers;

K- Kinasing DNA;

L - Vectors for suppressor rescue; Prep of DNA for binding nitrocellulose filters, Plasmid preps, C. Lysis, Extraction of lambda phage DNA

Scope and Contents

MN - Making template MI3 Radioactive labeling of DNA by Nick Translation Northern procedure Nitrocellulose

OPQ - Cellulose phosphate ion exchanges, Phosphocellulose preparation, oligonucleotide workup procedure, Mark Zoller, Properties of some standard proteins, Biorad protein assay, 1977, PUR 222 Plasmid prep, Plaque titration

Scope and Contents

R - Methods for superior RNA from tissue culture, 1980;

S - Storgae of plasmid colonies, southern blots, DNA sequencing gels;

T - Transformation protocol for e coli DHI

Scope and Contents From the Sub-Series: Laboratory materials from BW Stillman and others working in his laboratory. The sub-series consists of experiments, protocols, methods, technician notes, negatives and photographic prints. Binders from BW Stillman: labelled experiments 1976-1989, Archaeal DNA Replication, Chromatin notes, Yeast, SV40, Topo S, 293 S100, RFC Sequencing. Others: Fred Bunz, Lynn Borzillo, Katie Cronin, Andrei Chabes, Gerhard Cullman, Nancy Du, Elena Ejkova, Jessica Mitchell, Patrick Finnegan, WJ Heiger,...