Box 05

Scope and Contents The notebook begins with a graph of the counts per minute of T2G4 oligonucleotide and the same nucleotide combined with pBR enzyme. Greider then ran a program on the corresponding gel. Like previous notebooks she reviewed and remarked on invitro experiments (T2G4). Also sets up two kinds of experiments: One is a processivity experiment: mix enzyme with T2G4 and combined (T2G4 + buffer). The second is a Competition experiment: mixes enzyme with T2G4 and combined (T2G4 + ddT). Greider had a...

Scope and Contents

The notebook has a list of things to do, from gel purification to dyeing techniques. Also has a protocal for purification for Synthetic Oligonucleotides and gel purification and of specific columns, like C18 column. Graphed the data, highlighting oligonucletides in the graph, and enzymatic action. Greider concluded the notebook with a series of gel electrophoresis studies.

Scope and Contents The notebook begins with a general expression of telomerase RNA through in vitro experiments without extraneous nucleotides.Also Greider discussed Polymerase Chain Reaction cycles. The cyles are denaturing the protein, spinning down to a nucleate, loading the gel. Greider then began to dye the gel.Greider was able to draw out the universal primer and reverse primer from the gel. However, found no new mutations in the composite sequence of both. Greider...

Scope and Contents Begins with a Tetrahymena ribosmoal DNA subsequence series and corresponding restriction enzyme Library Map Also has a DNA Oligomer Request Form by Greider (18 oligomer) of nucleotides. Thymadine and Guanine. She ran more Southern blots and cut more Tet DNA for blots. Then Depurified blot gels and hybridized all 5 blots at 45 degrees Celcius. However she realized that she should have added a probe before hybradizing. Conducts several ligation tests using Hind enzyme and several yeast blot...