Box 04

Scope and Contents The notebook begins with Greider treating RNA strands with a MNase enzyme, which split the strand in half. Greider then ran the RNA strands through a gel, thereby creating six ladders of DNA each with corresponding bands and graphed the sensitivity of MNase onto the RNA strand. Then Greider tested the MNase enzyme on a sodium chloride compound and DNA. Greider found that there was something in the DNA and RNA strands that seemed to inhibit the MNase enzyme. After conducting the...

Scope and Contents The notebook begins with diagrams of RNA structure. The structure noted due to abundance of Urasil nucleotide. Greider also constructed diagrams of elongation of C4A2, its translocation with G4T2 and then subsequent elongation. Greider reviewed her previous RNA and invitro experimentsand fractionalization studies. Grieder also remarked on each experiment, and made notes on which ones involved gels and titrations.In group meeting, Greider was still working on reconstitution study...