Cloning cDNAs

The notebook contains films created from the Blackburn laboratory with x-rays inside the notebook. The notebook begins with x-rays of colony's of hybrids that Grieder bred in order to extract their cDNA. The next set in the file, used tRNA with titration studies. The RNA being used is for cloning. The next gel used restriction enzymes, PUC119, for certain nucleotides over a thirty-six hour exposure on an open screen. The gels that were done used RNA, cDNA, oligonucleotides with Grieder marking a 1kb marker. The next gels were quite interesting as Grieder labels which DNA cut and uncut and then labelled with RTase. In another gel, labelled Plan B, Grieder used a significant amount of cDNA and RNA, which was noted by the abundance of Uracil. The next set focused of gels with full DNA sequences. The next gels were done with no screen at all and produced no columns. After synthesizing the second strand from previous gels. The last section concluded with several mistakes that she made due to the degradation of the DNA.