CWG Films IV 73-104

The notebook contains films created from the Blackburn laboratory with x-rays inside the notebook. This notebook, Grieder has xrayed several gel elctrophoresis experiments. The first in this file is IV 73. In IV 73, Grieder conducts two seperate experiments. One is for an eiht day exposure and the other using an MNase enzyme and a single stranded RNA sequence onto an open screen. The other was exposed for twenty-four hours using an RNA strand on an open screen. Bothe xperiments produced significantly dark bands in several columns. IV 74 was similar in many respects to IV 73, in that it also had multiple experiments. Overall the exposure was around 18 to 20 hours for both experiments. An interesting note in the second gel experiment, Grieder noted a certain DNA bands within a series of columns and noted it with a question mark. In IV 75, Grieder used RNase enzyme in the gel and noted the production of crude and new oligonucleotides in the gel. The next folder in the file is labeled onot in with a standard IV indentifier bt with the label, Limited Nucleases for a series of IV experriments, namely IV 74- IV 76. These two used two differnet enzymes, RNase and MNase, for both sides of the gel's columns. Each was exposed for a variable number of times. In IV 80, The most significant aspect of the series of gels is Grieder's use of repeated DNA sequences, namely T2G4. C4A2, and C4A4. In IV 81, Grieder added CT DNA in certain columns instead of RNA. In IV 82, Grieder measure sthe tmperature of certain columns of DNA as they are run through the gel. In IV 83, the gel used DNA extracted using ddTTP. The exact DNA sequence was a DNA repeat (G4T2)2 with an exposed screen. In IV 84, Grieder labelled the Tymaine and the Guanine in each of the columns. Each experiment in that file was exposed for 13 days. In IV 85, Grieder wrote a note to specifcally expose certain columns for certain amount oftime in order to create lighter bands. Iv 87, 89, and 90 were standard gel experiments with the subsequent lighter and darker bands characteristic of normal exposure. IV 94 was curious beacuse its used a repeating DNA sequence, specifcally (T2G4)4. IV 95 was ot only a gel electrophoresis experiment but also a fractionalization experiment as well with Grieder noting columns of DNA that used specific enzymes. IV 98 was quite curious as well. None of the experiments produced a standard gel or column. It could be that the enzyme that was used to break up the DNA dissolved it. Except for the last gel study in the file, which Grieder labelled as having havy amount of Guanine and Thymine. In IV 100 experiments, Grider diluted the DNA over multiple mintes to see how they reacted and also diluted it in a cold reaction down to the millemeter. In 101 and 102, the level of analysis and detail increase compared to previous experiments. In that, Grieder analyzed the ratio of Uracil and Thymadine in single stranded RNA sequences that were fed through the gel. The concluding invitro experiment IV 104, the (T2G4)4 and mixed the sequence with (G4T2)3 all of which were exposed.