CWG Films IV 37-72

The notebook contains films created from the Blackburn laboratory with x-rays inside the notebook. The notebook begins with IV 37, which used DNA remnants with a 2 day exposure. The gel resulted in very dark bands for all of the columns. IV 40 the gel was exposed for four days, the gel labelled with salt (salt chloride) and pottasium chloride and with open screen. The other side of IV 40, the gel was exposed over sixteen hours and open screen. In IV 41, one gel was a constant dark band but interestingly enough the gel columns were not present. It was exposed over six days. Another inresting note was that the bottom of the gel was amalgamated. On the other side of the same gel, the top of the gel was dark and exposed. The first column was fully darkened. In IV 42, there was a two week exposure producing four distinct dark bands. On the other side, the Cytosine-Thymine nuceotide DNA bands were the darkest. Two day exposure and open screen. A dialyzed gel was exposed for five days with an open screen. In IV 44, there was a two week exposure with an open screen. Slight dark and light bands interspersed in the gel. In IV 45, it was a one and half day exposure with an open screen. One part of the gel used extracts and other used dialysis. In IV 47, the document is interesting because a twenty four hour exposure did not create dark bands but was degraded, possibly due to exposure. IV 48, was also exposed for twenty-four hours, however the gel produced light bands and columns. Obviously the one with no oligonucleotides had virtually no distinctive columns or bands of DNA. The same is true for IV 49 however IV 52 was exposed for two and a half days with two different enzymes: Proteinase A and RNAse A and with an open screen. An interesting note is in IV 55 which was exposed on an open screen for 2.5 days with both live RNAse and dead RNAse extracts. This gel was very similar to the results found in IV 59. IV 61 was done with a three different DNA sequences, G4T2, Yeast DNA, and C4A2. With the final column having no oligonucleotides. It was exposed over three days with an open screen. Other invitro experiments was done with preincubated oligonucleotides. Iv 64 was exposed for a whole month creating very dark bands ad columns of DNA. IV 71 was split into two different gel studies. The first was done over seven days with an open screen. The second is used RNA 13 with a 1 kilobase marker and with a certain column having a tRNA. IV 71 had a total of four invitro experiments with the last gel coming from a colleague of Grieder’s, James.