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CWG Films IV 3-14

 File — Case: 07_001
Identifier: CWG_07_001
CWG/7/1 Film IV 3-14
CWG/7/1 Film IV 3-14

Scope and Contents

The notebook begins with a gel that has a darker exposure. The light exposure in IV 4, where a certain DNA sequence was used, utilized a Taq restriction enzyme over seven days. In another gel, there was exposure with distinct markers, specifically using Taq and Kinase as restriction enzymes. In IV 5, Grieder loaded two different gels with different fragments and restriction enzymes. In IV 6, Grieder exposed the gel for three days on a screen and in IV 7, there was a seven day exposure on a specific DNA sequence, G4T2. In IV 10, Grieder used a 6% Acryl gel that was set and the DNA was poured through over 24 hour exposures. Grieder did another gel with a 3 week exposure. In IV 11, used a 6 day exposure for all gels in this particular invitro experiment. In IV 13, used a seventeen day exposure resulting in dark columns under a Urea gel. In another gel, Grieder exposed it over six days with the combination of Yeast Oligonucleotide, an MNase enzyme, and cell extacts. Invitro experiment 14 used a Urea gel on one side and Acryl gel 6% with four day exposure.

Dates

  • Creation: 1983 - 1995

Creator

Conditions Governing Access

No restictions apply to this collection. Access is given only by appointment, 9 a.m. to 4:30 p.m. Monday through Friday.

Extent

From the Collection: 34 Boxes : 30 Linear Feet. The majority of this collection has been digitized. Digital images can be viewed by clicking links within this online finding aid. ; 8.75 linear fee/notebooks, 19.83 LF/films

Language of Materials

From the Collection: English

File Plan

Lab Notebooks

Repository Details

Part of the Cold Spring Harbor Laboratory Archives Repository

Contact:
Library & Archives
Cold Spring Harbor Laboratory
One Bungtown Rd
Cold Spring Harbor NY 11724 USA
516-367-6872