Laboratory Notebook: TLE, Early Notebooks/Telomere Cloning, 1984 - 1992

The notebook begins with an interesting note: Greider was taking classes in Biochemistry in 1984. Proceeded right back into experiments. Diluting and spinning cells into pellets and running minipreps. Also is spheroplasting cells, meaning Greider was destroying the outside cell wall with enzyme zymolyase. Greider spun down the nucleate and ran RNAse with nucleate through a gel. Running more gel studies, she found two bands. They seem to be upper and lower plasmid bands. Greider wanted to cut out each one using glass beads. The possibility for there being two bands might be that there are two plasmids there. Greider found that both then are telomeric fragments, or end fragments of differnt sizes. The overall goal of these experiments were to find cells with plasmids that hybridize strongly. She runs agarose gels and modify’s the cloning techniques slightly.