Laboratory Notebook: "RNA Oligo" Blots, Cloning DCG1, 1987 - 1988

File — Box: 05, Folder: 05

Identifier: CWG_01_021

Staff Only

CWG-01-021 RNA Oligo blots Cloning

Scope and Contents

Begins with a Tetrahymena ribosmoal DNA subsequence series and corresponding restriction enzyme Library Map Also has a DNA Oligomer Request Form by Greider (18 oligomer) of nucleotides. Thymadine and Guanine. She ran more Southern blots and cut more Tet DNA for blots. Then Depurified blot gels and hybridized all 5 blots at 45 degrees Celcius. However she realized that she should have added a probe before hybradizing. Conducts several ligation tests using Hind enzyme and several yeast blot tests. Documents of note is a correspondence from Garnet at Berkley to Greider, describing his cell colony, treated it through denaturing, drying, and neutralizing the colony.

Dates

Creation: 1987 - 1988

Creator

From the Collection: Greider, Carol W. (Author, Person)
From the Collection: University of California, Berkeley (Laboratory, Organization)
From the Collection: Harley, Calvin (Author, Person)

Conditions Governing Access

No restictions apply to this collection. Access is given only by appointment, 9 a.m. to 4:30 p.m. Monday through Friday.

Extent

From the Collection: 34 Boxes : 30 Linear Feet. The majority of this collection has been digitized. Digital images can be viewed by clicking links within this online finding aid. ; 8.75 linear fee/notebooks, 19.83 LF/films