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Laboratory Notebook 4: In Vitro 56-80/FR 20-40/RNA 10-21, 1985 - 1986

 File — Box: 02, Folder: 03-04
Identifier: CWG_01_007
CWG-01-007 IV 56-80
CWG-01-007 IV 56-80

Scope and Contents

This is a notebook within a series regarding experiments that Greider had done at UC Berkeley. The notebook begins with a summary of experiments that Greider had done so far. Greider set up an experiment with mated, spun, and lysed cells. Greider then mixed the cells to create a pellet of nucleate. With the nucleate Greider began testing different extracts for it and set up reactants, specifcally, G4T2 and NTP. Then ran the product through the gel and the bands were fuzzy. In two particular invitro experiments, Greider tested both hot and cold rNTP’s and fit them into tubes and then ran the tubes through a titration.

Greider found that the rNTP was not well incorporated into the base pair pattern of nucleotides in the DNA segement. She began testing different possibilities in order to figure out what the rNTP is doing in the process. In the end Greider ran the segment through a gel. Greider loaded a different gel this time using specific extracts, reactions, nucleotides. After the experiment, Greider tried to determine how much excess DNA she was adding. She concluded the notebook by reviewing the different preparations to extract DNA. Greider showcased the measurements of buffer solutions, reactants, cell mating processes, and suspension.

Dates

  • Creation: 1985 - 1986

Creator

Conditions Governing Access

No restictions apply to this collection. Access is given only by appointment, 9 a.m. to 4:30 p.m. Monday through Friday.

Extent

2 Folders

Language of Materials

From the Collection: English

File Plan

Lab Notebooks

File Plan

Clippings

File Plan

Correspondence

Repository Details

Part of the Cold Spring Harbor Laboratory Archives Repository

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